pmd g vsvg addgene Search Results


vsv g expression plasmid pmd g  (Addgene inc)
98
Addgene inc vsv g expression plasmid pmd g
Vsv G Expression Plasmid Pmd G, supplied by Addgene inc, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pmd+g+vsvg+addgene/pm34246112-173-27-32?v=Addgene+inc
Average 98 stars, based on 1 article reviews
vsv g expression plasmid pmd g - by Bioz Stars, 2026-07
98/100 stars
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pmd gvsv g  (Addgene inc)
96
Addgene inc pmd gvsv g
Pmd Gvsv G, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pmd+g+vsvg+addgene/pm39383211-71-14-23?v=Addgene+inc
Average 96 stars, based on 1 article reviews
pmd gvsv g - by Bioz Stars, 2026-07
96/100 stars
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type wt vesicular stomatitis virus glycoprotein  (Addgene inc)
96
Addgene inc type wt vesicular stomatitis virus glycoprotein
(A) The panel depicts the cellularly expressed Spike protein in blue, with RBD regions as horseshoe shapes. The viral membrane is depicted as pseudotyped with the Tupaia paramyxovirus (TPMV) fusion (F) protein (yellow), associated with a H-Nano chimera of the binding-defective hemaglutinin (H) protein (green) and a nanobody (Nano; red) that binds to the Spike RBD. In this model, binding of the H-Nano protein to Spike activates the TPMV F protein to fuse the viral and cell membranes. (B) The panel depicts an alternative model, in which binding of the viral H-Nano protein to the cellular Spike protein activates Spike to fuse membranes in a TPMV F-independent fashion. (C) β-galactosidase (βGal) reporter lentiviruses were pseudotyped with the binding-defective TPMV H protein plus the TPMV F protein (H+F), or the H-Nano chimeric protein plus F (H-Nano + F), or the H-Nano protein alone (H-Nano). The viruses were used to infect Spike-expressing cells. Infection levels were measured via βGal activities and were normalized to the H-Nano + F levels. Averages and standard deviations are as shown. Note that the H-Nano+F and H-Nano infection levels both were significantly higher than the H+N control (P<0.001). (D) Spike-positive (Spike+) or Spike-negative (Spike-) cells were infected with βGal lentivirus vectors pseudotyped with the wild type (WT) Vesicular <t>stomatitis</t> virus <t>glycoprotein</t> (VSV G), or H-Nano + F, or H-Nano alone. Infection levels for each pseudotyped virus were normalized to infection of the Spike+ cells, and averages and standard deviations are as shown. Note that the difference for the H-Nano virus between Spike+ and Spike-cells was highly significant (P<0.001), while the difference for H-Nano + F was significant (P=0.002).
Type Wt Vesicular Stomatitis Virus Glycoprotein, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pmd+g+vsvg+addgene/bio_rxiv__2024__06__06__597774-115-2-48?v=Addgene+inc
Average 96 stars, based on 1 article reviews
type wt vesicular stomatitis virus glycoprotein - by Bioz Stars, 2026-07
96/100 stars
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pmdg vsvg  (Addgene inc)
93
Addgene inc pmdg vsvg
(A) The panel depicts the cellularly expressed Spike protein in blue, with RBD regions as horseshoe shapes. The viral membrane is depicted as pseudotyped with the Tupaia paramyxovirus (TPMV) fusion (F) protein (yellow), associated with a H-Nano chimera of the binding-defective hemaglutinin (H) protein (green) and a nanobody (Nano; red) that binds to the Spike RBD. In this model, binding of the H-Nano protein to Spike activates the TPMV F protein to fuse the viral and cell membranes. (B) The panel depicts an alternative model, in which binding of the viral H-Nano protein to the cellular Spike protein activates Spike to fuse membranes in a TPMV F-independent fashion. (C) β-galactosidase (βGal) reporter lentiviruses were pseudotyped with the binding-defective TPMV H protein plus the TPMV F protein (H+F), or the H-Nano chimeric protein plus F (H-Nano + F), or the H-Nano protein alone (H-Nano). The viruses were used to infect Spike-expressing cells. Infection levels were measured via βGal activities and were normalized to the H-Nano + F levels. Averages and standard deviations are as shown. Note that the H-Nano+F and H-Nano infection levels both were significantly higher than the H+N control (P<0.001). (D) Spike-positive (Spike+) or Spike-negative (Spike-) cells were infected with βGal lentivirus vectors pseudotyped with the wild type (WT) Vesicular <t>stomatitis</t> virus <t>glycoprotein</t> (VSV G), or H-Nano + F, or H-Nano alone. Infection levels for each pseudotyped virus were normalized to infection of the Spike+ cells, and averages and standard deviations are as shown. Note that the difference for the H-Nano virus between Spike+ and Spike-cells was highly significant (P<0.001), while the difference for H-Nano + F was significant (P=0.002).
Pmdg Vsvg, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pmd+g+vsvg+addgene/pmc06701927-326-26-32?v=Addgene+inc
Average 93 stars, based on 1 article reviews
pmdg vsvg - by Bioz Stars, 2026-07
93/100 stars
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pmd g vsv g  (Addgene inc)
96
Addgene inc pmd g vsv g
(A) The panel depicts the cellularly expressed Spike protein in blue, with RBD regions as horseshoe shapes. The viral membrane is depicted as pseudotyped with the Tupaia paramyxovirus (TPMV) fusion (F) protein (yellow), associated with a H-Nano chimera of the binding-defective hemaglutinin (H) protein (green) and a nanobody (Nano; red) that binds to the Spike RBD. In this model, binding of the H-Nano protein to Spike activates the TPMV F protein to fuse the viral and cell membranes. (B) The panel depicts an alternative model, in which binding of the viral H-Nano protein to the cellular Spike protein activates Spike to fuse membranes in a TPMV F-independent fashion. (C) β-galactosidase (βGal) reporter lentiviruses were pseudotyped with the binding-defective TPMV H protein plus the TPMV F protein (H+F), or the H-Nano chimeric protein plus F (H-Nano + F), or the H-Nano protein alone (H-Nano). The viruses were used to infect Spike-expressing cells. Infection levels were measured via βGal activities and were normalized to the H-Nano + F levels. Averages and standard deviations are as shown. Note that the H-Nano+F and H-Nano infection levels both were significantly higher than the H+N control (P<0.001). (D) Spike-positive (Spike+) or Spike-negative (Spike-) cells were infected with βGal lentivirus vectors pseudotyped with the wild type (WT) Vesicular <t>stomatitis</t> virus <t>glycoprotein</t> (VSV G), or H-Nano + F, or H-Nano alone. Infection levels for each pseudotyped virus were normalized to infection of the Spike+ cells, and averages and standard deviations are as shown. Note that the difference for the H-Nano virus between Spike+ and Spike-cells was highly significant (P<0.001), while the difference for H-Nano + F was significant (P=0.002).
Pmd G Vsv G, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pmd+g+vsvg+addgene/pmc06978389-39-20-30?v=Addgene+inc
Average 96 stars, based on 1 article reviews
pmd g vsv g - by Bioz Stars, 2026-07
96/100 stars
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pmdg  (GenScript corporation)
90
GenScript corporation pmdg
(A) The panel depicts the cellularly expressed Spike protein in blue, with RBD regions as horseshoe shapes. The viral membrane is depicted as pseudotyped with the Tupaia paramyxovirus (TPMV) fusion (F) protein (yellow), associated with a H-Nano chimera of the binding-defective hemaglutinin (H) protein (green) and a nanobody (Nano; red) that binds to the Spike RBD. In this model, binding of the H-Nano protein to Spike activates the TPMV F protein to fuse the viral and cell membranes. (B) The panel depicts an alternative model, in which binding of the viral H-Nano protein to the cellular Spike protein activates Spike to fuse membranes in a TPMV F-independent fashion. (C) β-galactosidase (βGal) reporter lentiviruses were pseudotyped with the binding-defective TPMV H protein plus the TPMV F protein (H+F), or the H-Nano chimeric protein plus F (H-Nano + F), or the H-Nano protein alone (H-Nano). The viruses were used to infect Spike-expressing cells. Infection levels were measured via βGal activities and were normalized to the H-Nano + F levels. Averages and standard deviations are as shown. Note that the H-Nano+F and H-Nano infection levels both were significantly higher than the H+N control (P<0.001). (D) Spike-positive (Spike+) or Spike-negative (Spike-) cells were infected with βGal lentivirus vectors pseudotyped with the wild type (WT) Vesicular <t>stomatitis</t> virus <t>glycoprotein</t> (VSV G), or H-Nano + F, or H-Nano alone. Infection levels for each pseudotyped virus were normalized to infection of the Spike+ cells, and averages and standard deviations are as shown. Note that the difference for the H-Nano virus between Spike+ and Spike-cells was highly significant (P<0.001), while the difference for H-Nano + F was significant (P=0.002).
Pmdg, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pmd+g+vsvg+addgene/bio_rxiv__838011-172-41-48?v=GenScript+corporation
Average 90 stars, based on 1 article reviews
pmdg - by Bioz Stars, 2026-07
90/100 stars
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vesicular stomatitis virus g glycoprotein expressing plasmid pmdg  (GenScript corporation)
90
GenScript corporation vesicular stomatitis virus g glycoprotein expressing plasmid pmdg
(A) The panel depicts the cellularly expressed Spike protein in blue, with RBD regions as horseshoe shapes. The viral membrane is depicted as pseudotyped with the Tupaia paramyxovirus (TPMV) fusion (F) protein (yellow), associated with a H-Nano chimera of the binding-defective hemaglutinin (H) protein (green) and a nanobody (Nano; red) that binds to the Spike RBD. In this model, binding of the H-Nano protein to Spike activates the TPMV F protein to fuse the viral and cell membranes. (B) The panel depicts an alternative model, in which binding of the viral H-Nano protein to the cellular Spike protein activates Spike to fuse membranes in a TPMV F-independent fashion. (C) β-galactosidase (βGal) reporter lentiviruses were pseudotyped with the binding-defective TPMV H protein plus the TPMV F protein (H+F), or the H-Nano chimeric protein plus F (H-Nano + F), or the H-Nano protein alone (H-Nano). The viruses were used to infect Spike-expressing cells. Infection levels were measured via βGal activities and were normalized to the H-Nano + F levels. Averages and standard deviations are as shown. Note that the H-Nano+F and H-Nano infection levels both were significantly higher than the H+N control (P<0.001). (D) Spike-positive (Spike+) or Spike-negative (Spike-) cells were infected with βGal lentivirus vectors pseudotyped with the wild type (WT) Vesicular <t>stomatitis</t> virus <t>glycoprotein</t> (VSV G), or H-Nano + F, or H-Nano alone. Infection levels for each pseudotyped virus were normalized to infection of the Spike+ cells, and averages and standard deviations are as shown. Note that the difference for the H-Nano virus between Spike+ and Spike-cells was highly significant (P<0.001), while the difference for H-Nano + F was significant (P=0.002).
Vesicular Stomatitis Virus G Glycoprotein Expressing Plasmid Pmdg, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pmd+g+vsvg+addgene/pmc07560218-215-43-50?v=GenScript+corporation
Average 90 stars, based on 1 article reviews
vesicular stomatitis virus g glycoprotein expressing plasmid pmdg - by Bioz Stars, 2026-07
90/100 stars
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pcmv deltar8 2  (Addgene inc)
94
Addgene inc pcmv deltar8 2
(A) The panel depicts the cellularly expressed Spike protein in blue, with RBD regions as horseshoe shapes. The viral membrane is depicted as pseudotyped with the Tupaia paramyxovirus (TPMV) fusion (F) protein (yellow), associated with a H-Nano chimera of the binding-defective hemaglutinin (H) protein (green) and a nanobody (Nano; red) that binds to the Spike RBD. In this model, binding of the H-Nano protein to Spike activates the TPMV F protein to fuse the viral and cell membranes. (B) The panel depicts an alternative model, in which binding of the viral H-Nano protein to the cellular Spike protein activates Spike to fuse membranes in a TPMV F-independent fashion. (C) β-galactosidase (βGal) reporter lentiviruses were pseudotyped with the binding-defective TPMV H protein plus the TPMV F protein (H+F), or the H-Nano chimeric protein plus F (H-Nano + F), or the H-Nano protein alone (H-Nano). The viruses were used to infect Spike-expressing cells. Infection levels were measured via βGal activities and were normalized to the H-Nano + F levels. Averages and standard deviations are as shown. Note that the H-Nano+F and H-Nano infection levels both were significantly higher than the H+N control (P<0.001). (D) Spike-positive (Spike+) or Spike-negative (Spike-) cells were infected with βGal lentivirus vectors pseudotyped with the wild type (WT) Vesicular <t>stomatitis</t> virus <t>glycoprotein</t> (VSV G), or H-Nano + F, or H-Nano alone. Infection levels for each pseudotyped virus were normalized to infection of the Spike+ cells, and averages and standard deviations are as shown. Note that the difference for the H-Nano virus between Spike+ and Spike-cells was highly significant (P<0.001), while the difference for H-Nano + F was significant (P=0.002).
Pcmv Deltar8 2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pmd+g+vsvg+addgene/bio_rxiv__2023__12__11__571135-123-52-60?v=Addgene+inc
Average 94 stars, based on 1 article reviews
pcmv deltar8 2 - by Bioz Stars, 2026-07
94/100 stars
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fugene 6 transfection reagent  (Promega)
90
Promega fugene 6 transfection reagent
(A) The panel depicts the cellularly expressed Spike protein in blue, with RBD regions as horseshoe shapes. The viral membrane is depicted as pseudotyped with the Tupaia paramyxovirus (TPMV) fusion (F) protein (yellow), associated with a H-Nano chimera of the binding-defective hemaglutinin (H) protein (green) and a nanobody (Nano; red) that binds to the Spike RBD. In this model, binding of the H-Nano protein to Spike activates the TPMV F protein to fuse the viral and cell membranes. (B) The panel depicts an alternative model, in which binding of the viral H-Nano protein to the cellular Spike protein activates Spike to fuse membranes in a TPMV F-independent fashion. (C) β-galactosidase (βGal) reporter lentiviruses were pseudotyped with the binding-defective TPMV H protein plus the TPMV F protein (H+F), or the H-Nano chimeric protein plus F (H-Nano + F), or the H-Nano protein alone (H-Nano). The viruses were used to infect Spike-expressing cells. Infection levels were measured via βGal activities and were normalized to the H-Nano + F levels. Averages and standard deviations are as shown. Note that the H-Nano+F and H-Nano infection levels both were significantly higher than the H+N control (P<0.001). (D) Spike-positive (Spike+) or Spike-negative (Spike-) cells were infected with βGal lentivirus vectors pseudotyped with the wild type (WT) Vesicular <t>stomatitis</t> virus <t>glycoprotein</t> (VSV G), or H-Nano + F, or H-Nano alone. Infection levels for each pseudotyped virus were normalized to infection of the Spike+ cells, and averages and standard deviations are as shown. Note that the difference for the H-Nano virus between Spike+ and Spike-cells was highly significant (P<0.001), while the difference for H-Nano + F was significant (P=0.002).
Fugene 6 Transfection Reagent, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pmd+g+vsvg+addgene/bio_rxiv__838011-172-50-54?v=Promega
Average 90 stars, based on 1 article reviews
fugene 6 transfection reagent - by Bioz Stars, 2026-07
90/100 stars
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guide rnas  (Addgene inc)
96
Addgene inc guide rnas
(A) The panel depicts the cellularly expressed Spike protein in blue, with RBD regions as horseshoe shapes. The viral membrane is depicted as pseudotyped with the Tupaia paramyxovirus (TPMV) fusion (F) protein (yellow), associated with a H-Nano chimera of the binding-defective hemaglutinin (H) protein (green) and a nanobody (Nano; red) that binds to the Spike RBD. In this model, binding of the H-Nano protein to Spike activates the TPMV F protein to fuse the viral and cell membranes. (B) The panel depicts an alternative model, in which binding of the viral H-Nano protein to the cellular Spike protein activates Spike to fuse membranes in a TPMV F-independent fashion. (C) β-galactosidase (βGal) reporter lentiviruses were pseudotyped with the binding-defective TPMV H protein plus the TPMV F protein (H+F), or the H-Nano chimeric protein plus F (H-Nano + F), or the H-Nano protein alone (H-Nano). The viruses were used to infect Spike-expressing cells. Infection levels were measured via βGal activities and were normalized to the H-Nano + F levels. Averages and standard deviations are as shown. Note that the H-Nano+F and H-Nano infection levels both were significantly higher than the H+N control (P<0.001). (D) Spike-positive (Spike+) or Spike-negative (Spike-) cells were infected with βGal lentivirus vectors pseudotyped with the wild type (WT) Vesicular <t>stomatitis</t> virus <t>glycoprotein</t> (VSV G), or H-Nano + F, or H-Nano alone. Infection levels for each pseudotyped virus were normalized to infection of the Spike+ cells, and averages and standard deviations are as shown. Note that the difference for the H-Nano virus between Spike+ and Spike-cells was highly significant (P<0.001), while the difference for H-Nano + F was significant (P=0.002).
Guide Rnas, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pmd+g+vsvg+addgene/pmc07560218-215-23-25?v=Addgene+inc
Average 96 stars, based on 1 article reviews
guide rnas - by Bioz Stars, 2026-07
96/100 stars
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simon davis  (Addgene inc)
93
Addgene inc simon davis
(A) The panel depicts the cellularly expressed Spike protein in blue, with RBD regions as horseshoe shapes. The viral membrane is depicted as pseudotyped with the Tupaia paramyxovirus (TPMV) fusion (F) protein (yellow), associated with a H-Nano chimera of the binding-defective hemaglutinin (H) protein (green) and a nanobody (Nano; red) that binds to the Spike RBD. In this model, binding of the H-Nano protein to Spike activates the TPMV F protein to fuse the viral and cell membranes. (B) The panel depicts an alternative model, in which binding of the viral H-Nano protein to the cellular Spike protein activates Spike to fuse membranes in a TPMV F-independent fashion. (C) β-galactosidase (βGal) reporter lentiviruses were pseudotyped with the binding-defective TPMV H protein plus the TPMV F protein (H+F), or the H-Nano chimeric protein plus F (H-Nano + F), or the H-Nano protein alone (H-Nano). The viruses were used to infect Spike-expressing cells. Infection levels were measured via βGal activities and were normalized to the H-Nano + F levels. Averages and standard deviations are as shown. Note that the H-Nano+F and H-Nano infection levels both were significantly higher than the H+N control (P<0.001). (D) Spike-positive (Spike+) or Spike-negative (Spike-) cells were infected with βGal lentivirus vectors pseudotyped with the wild type (WT) Vesicular <t>stomatitis</t> virus <t>glycoprotein</t> (VSV G), or H-Nano + F, or H-Nano alone. Infection levels for each pseudotyped virus were normalized to infection of the Spike+ cells, and averages and standard deviations are as shown. Note that the difference for the H-Nano virus between Spike+ and Spike-cells was highly significant (P<0.001), while the difference for H-Nano + F was significant (P=0.002).
Simon Davis, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pmd+g+vsvg+addgene/pm39016538-376-72-74?v=Addgene+inc
Average 93 stars, based on 1 article reviews
simon davis - by Bioz Stars, 2026-07
93/100 stars
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hiv 1 packing plasmid encoding gfp  (Addgene inc)
92
Addgene inc hiv 1 packing plasmid encoding gfp
(A) The panel depicts the cellularly expressed Spike protein in blue, with RBD regions as horseshoe shapes. The viral membrane is depicted as pseudotyped with the Tupaia paramyxovirus (TPMV) fusion (F) protein (yellow), associated with a H-Nano chimera of the binding-defective hemaglutinin (H) protein (green) and a nanobody (Nano; red) that binds to the Spike RBD. In this model, binding of the H-Nano protein to Spike activates the TPMV F protein to fuse the viral and cell membranes. (B) The panel depicts an alternative model, in which binding of the viral H-Nano protein to the cellular Spike protein activates Spike to fuse membranes in a TPMV F-independent fashion. (C) β-galactosidase (βGal) reporter lentiviruses were pseudotyped with the binding-defective TPMV H protein plus the TPMV F protein (H+F), or the H-Nano chimeric protein plus F (H-Nano + F), or the H-Nano protein alone (H-Nano). The viruses were used to infect Spike-expressing cells. Infection levels were measured via βGal activities and were normalized to the H-Nano + F levels. Averages and standard deviations are as shown. Note that the H-Nano+F and H-Nano infection levels both were significantly higher than the H+N control (P<0.001). (D) Spike-positive (Spike+) or Spike-negative (Spike-) cells were infected with βGal lentivirus vectors pseudotyped with the wild type (WT) Vesicular <t>stomatitis</t> virus <t>glycoprotein</t> (VSV G), or H-Nano + F, or H-Nano alone. Infection levels for each pseudotyped virus were normalized to infection of the Spike+ cells, and averages and standard deviations are as shown. Note that the difference for the H-Nano virus between Spike+ and Spike-cells was highly significant (P<0.001), while the difference for H-Nano + F was significant (P=0.002).
Hiv 1 Packing Plasmid Encoding Gfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pmd+g+vsvg+addgene/pmc11320578-304-31-60?v=Addgene+inc
Average 92 stars, based on 1 article reviews
hiv 1 packing plasmid encoding gfp - by Bioz Stars, 2026-07
92/100 stars
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Image Search Results


(A) The panel depicts the cellularly expressed Spike protein in blue, with RBD regions as horseshoe shapes. The viral membrane is depicted as pseudotyped with the Tupaia paramyxovirus (TPMV) fusion (F) protein (yellow), associated with a H-Nano chimera of the binding-defective hemaglutinin (H) protein (green) and a nanobody (Nano; red) that binds to the Spike RBD. In this model, binding of the H-Nano protein to Spike activates the TPMV F protein to fuse the viral and cell membranes. (B) The panel depicts an alternative model, in which binding of the viral H-Nano protein to the cellular Spike protein activates Spike to fuse membranes in a TPMV F-independent fashion. (C) β-galactosidase (βGal) reporter lentiviruses were pseudotyped with the binding-defective TPMV H protein plus the TPMV F protein (H+F), or the H-Nano chimeric protein plus F (H-Nano + F), or the H-Nano protein alone (H-Nano). The viruses were used to infect Spike-expressing cells. Infection levels were measured via βGal activities and were normalized to the H-Nano + F levels. Averages and standard deviations are as shown. Note that the H-Nano+F and H-Nano infection levels both were significantly higher than the H+N control (P<0.001). (D) Spike-positive (Spike+) or Spike-negative (Spike-) cells were infected with βGal lentivirus vectors pseudotyped with the wild type (WT) Vesicular stomatitis virus glycoprotein (VSV G), or H-Nano + F, or H-Nano alone. Infection levels for each pseudotyped virus were normalized to infection of the Spike+ cells, and averages and standard deviations are as shown. Note that the difference for the H-Nano virus between Spike+ and Spike-cells was highly significant (P<0.001), while the difference for H-Nano + F was significant (P=0.002).

Journal: bioRxiv

Article Title: A Nanobody Interaction with SARS-CoV-2 Spike Allows the Versatile Targeting of Lentivirus Vectors

doi: 10.1101/2024.06.06.597774

Figure Lengend Snippet: (A) The panel depicts the cellularly expressed Spike protein in blue, with RBD regions as horseshoe shapes. The viral membrane is depicted as pseudotyped with the Tupaia paramyxovirus (TPMV) fusion (F) protein (yellow), associated with a H-Nano chimera of the binding-defective hemaglutinin (H) protein (green) and a nanobody (Nano; red) that binds to the Spike RBD. In this model, binding of the H-Nano protein to Spike activates the TPMV F protein to fuse the viral and cell membranes. (B) The panel depicts an alternative model, in which binding of the viral H-Nano protein to the cellular Spike protein activates Spike to fuse membranes in a TPMV F-independent fashion. (C) β-galactosidase (βGal) reporter lentiviruses were pseudotyped with the binding-defective TPMV H protein plus the TPMV F protein (H+F), or the H-Nano chimeric protein plus F (H-Nano + F), or the H-Nano protein alone (H-Nano). The viruses were used to infect Spike-expressing cells. Infection levels were measured via βGal activities and were normalized to the H-Nano + F levels. Averages and standard deviations are as shown. Note that the H-Nano+F and H-Nano infection levels both were significantly higher than the H+N control (P<0.001). (D) Spike-positive (Spike+) or Spike-negative (Spike-) cells were infected with βGal lentivirus vectors pseudotyped with the wild type (WT) Vesicular stomatitis virus glycoprotein (VSV G), or H-Nano + F, or H-Nano alone. Infection levels for each pseudotyped virus were normalized to infection of the Spike+ cells, and averages and standard deviations are as shown. Note that the difference for the H-Nano virus between Spike+ and Spike-cells was highly significant (P<0.001), while the difference for H-Nano + F was significant (P=0.002).

Article Snippet: The wild type (WT) Vesicular stomatitis virus glycoprotein (VSV G) expression plasmid (pMD.G), the Tupaia paramyxovirus (TPMV) fusion and hemaglutinin expression plasmids (pCG-TPMV-Fd32, pCG-TPMV-Hd32), the SARS-CoV-2 Spike expression plasmid (CoV2-Spike-D614G), the green fluorescent protein expression plasmid (GFP-NoPS), and the lentivirus GagPol packaging plasmid (psPAX2) all were obtained from Addgene, and were the gifts of Simon Davis, Jakob Reiser, Jennifer Doudna, and Didier Trono ( , – ).

Techniques: Membrane, Binding Assay, Expressing, Infection, Control, Virus